Hold on there. Let's consider the positive controls for a second. Stefanoni had a lab standard called "STD 0.068", which I am assuming is a mix of known DNA template to a concentration of 68 picograms per milliliter. She would take 20 ml of this STD, and amplify via RT q-PCR, along with the rest of the field samples. She would then take the amplified standard and put it in the electrophoresis machine. She would get an egram. The egram should show a perfect profile of the of the lab standard.
But, what if we see extra stuff in the STD egram, i.e., peaks that don't belong to the STD donor? Doesn't that tell us that we have contamination? We shouldn't be able to see extra peaks, even if we magnify the egram all the way to the level of background noise, but what if we do?
I think that's why we don't have the STD egrams and/or the corresponding EDFs. Also, I think that's why we are missing a large number of egrams from plate no 365bis, which produced the egram for 36b (we have 757, 761, 770 and 771; are missing 758-760, and 762-769). Both the STD sample, and all of these missing samples effectively are controls, if we have the EDFS and can magnify them to the level that Stefanoni magnified 36b. What if they all produce artefacts and unknown secondary profiles? How reliable is plate 365bis?
Also, there might be negative controls, it's just that they might be at the beginning or end of the run sequences, in which case I haven't been able to identify them doing the gap analysis. Or, maybe Stefanoni thought that the STD control was good enough, for the reasons set forth above. IDK.