Chris_Halkides
Penultimate Amazing
- Joined
- Dec 8, 2009
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bra clasp DNA
I have reviewed some passages in the English translation of the Massei report that concern the bra clasp DNA. I would like to discuss these without revealing the reference profiles of any of the individuals.
On page 202 Stefanoni discussed her glove-changing practices, and these are not a rigorous as those delineated by John Butler, which I quoted earlier today. On page 206 Stefanoni used the word “noise” in a different manner from the way a spectroscopist would use it. From page 207, “Thus, where there is an allele which has a certain height and such that the peak just before it has a much smaller height, at most 15% of the first one, then the previous peak should be considered noise, just a by-product of the analysis.” One infers that Stefanoni used the word noise to refer to stutter peaks and possibly to refer to other artifacts such as blobs. Tagliabracci also used the word noise to mean stutter on p. 241.
On page 207 we read, “The height which is considered reliable for a peak to be qualified as an allele is equal to 50RFU, the symbol RFU representing the unit of measure employed for these measurements.” This is a remarkable statement in one respect, inasmuch as if Stefanoni actually adhered to it, about 22 of about 29 peaks attributed to Meredith on the knife profile would fail to be scored. In other words, she did not respect her own minimum threshold value in at least one other experiment.
On pages 208-209, one reads some strange statements. “She was asked if she had considered that peak, number 13 [This peak is in locus D5S818 and its height is 108 RFU], as an allele or as noise. Dr Stefanoni declared that she had not considered that peak as an allele or as noise… it can't be an allele because it is too low with respect to the main peaks.” This argument does not make sense; either a peak is an artifact or it is real. It is not in the correct position to be stutter, and there is no reason why a true allele can’t be smaller than Meredith’s profile. Moreover Tagliabracci questioned Stefanoni’s interpretation of this allele (pp. 241-242), noting that in locus vWA that she had taken a peak of only 65 RFU as an allele. Stefanoni argued that each peak should be judged on a case-by-case basis (p. 209), including information such as the main peak heights. Yet the main peaks in vWA are 84% as high as the main peaks in D5S818 on average, whereas 65 RFU is only 60% of 108 RFU. In other words what objective criterion Dr. Stefanoni used to reject a peak of 108 RFU and keep a peak of 65 RFU is obscure or nonexistent. Finally, there are other peaks in vWA at 17 and 18 repeats that are not labeled yet the larger of the two is about 50 RFU. It is difficult to see why this peak was not considered an allele.
Stefanoni was asked about an alternate interpretation, one in which a minor contributor would have the alleles 12 and 13. “In response to this question-observation, Dr Stefanoni explained that in this case, it would not be possible to explain the Y chromosome, and thus reaffirmed the correctness of the interpretation she had given.” It sounds as if Stefanoni used her attribution of the Y-chromosome profile to Raffaele to then interpret the autosomal DNA as having his profile. I would very much like to see what Conti and Vecchiotti have to say about this procedure.
Locus D21S11 is problematic (pp. 241-242). Stefanoni counted as stutter a peak that is 15.6% the height of the next peak. That is higher than the 15% cutoff, which is higher than stutter with which I am familiar. But she counted as real a peak that is 17.2% of the next peak, which is not that much of a difference. This peak could constitute half of Raffaele’s profile in this locus, but the other half would fall underneath one of Meredith’s two peaks, which are roughly sixfold higher. Although it is possible that Raffaele’s other allele is present and contributes additional intensity to the second of the two large peaks belonging to Meredith, I don’t see any reason to assume that it must.
To reiterate something I said earlier today, there are peaks on the bra clasp electropherogram that are not part of Raffaele’s profile and are not stutter, the nature of which I also discussed upthread. In reviewing the bra clasp DNA, I am more convinced than I was before that Stefanoni applied a suspect-centered approach.
I have reviewed some passages in the English translation of the Massei report that concern the bra clasp DNA. I would like to discuss these without revealing the reference profiles of any of the individuals.
On page 202 Stefanoni discussed her glove-changing practices, and these are not a rigorous as those delineated by John Butler, which I quoted earlier today. On page 206 Stefanoni used the word “noise” in a different manner from the way a spectroscopist would use it. From page 207, “Thus, where there is an allele which has a certain height and such that the peak just before it has a much smaller height, at most 15% of the first one, then the previous peak should be considered noise, just a by-product of the analysis.” One infers that Stefanoni used the word noise to refer to stutter peaks and possibly to refer to other artifacts such as blobs. Tagliabracci also used the word noise to mean stutter on p. 241.
On page 207 we read, “The height which is considered reliable for a peak to be qualified as an allele is equal to 50RFU, the symbol RFU representing the unit of measure employed for these measurements.” This is a remarkable statement in one respect, inasmuch as if Stefanoni actually adhered to it, about 22 of about 29 peaks attributed to Meredith on the knife profile would fail to be scored. In other words, she did not respect her own minimum threshold value in at least one other experiment.
On pages 208-209, one reads some strange statements. “She was asked if she had considered that peak, number 13 [This peak is in locus D5S818 and its height is 108 RFU], as an allele or as noise. Dr Stefanoni declared that she had not considered that peak as an allele or as noise… it can't be an allele because it is too low with respect to the main peaks.” This argument does not make sense; either a peak is an artifact or it is real. It is not in the correct position to be stutter, and there is no reason why a true allele can’t be smaller than Meredith’s profile. Moreover Tagliabracci questioned Stefanoni’s interpretation of this allele (pp. 241-242), noting that in locus vWA that she had taken a peak of only 65 RFU as an allele. Stefanoni argued that each peak should be judged on a case-by-case basis (p. 209), including information such as the main peak heights. Yet the main peaks in vWA are 84% as high as the main peaks in D5S818 on average, whereas 65 RFU is only 60% of 108 RFU. In other words what objective criterion Dr. Stefanoni used to reject a peak of 108 RFU and keep a peak of 65 RFU is obscure or nonexistent. Finally, there are other peaks in vWA at 17 and 18 repeats that are not labeled yet the larger of the two is about 50 RFU. It is difficult to see why this peak was not considered an allele.
Stefanoni was asked about an alternate interpretation, one in which a minor contributor would have the alleles 12 and 13. “In response to this question-observation, Dr Stefanoni explained that in this case, it would not be possible to explain the Y chromosome, and thus reaffirmed the correctness of the interpretation she had given.” It sounds as if Stefanoni used her attribution of the Y-chromosome profile to Raffaele to then interpret the autosomal DNA as having his profile. I would very much like to see what Conti and Vecchiotti have to say about this procedure.
Locus D21S11 is problematic (pp. 241-242). Stefanoni counted as stutter a peak that is 15.6% the height of the next peak. That is higher than the 15% cutoff, which is higher than stutter with which I am familiar. But she counted as real a peak that is 17.2% of the next peak, which is not that much of a difference. This peak could constitute half of Raffaele’s profile in this locus, but the other half would fall underneath one of Meredith’s two peaks, which are roughly sixfold higher. Although it is possible that Raffaele’s other allele is present and contributes additional intensity to the second of the two large peaks belonging to Meredith, I don’t see any reason to assume that it must.
To reiterate something I said earlier today, there are peaks on the bra clasp electropherogram that are not part of Raffaele’s profile and are not stutter, the nature of which I also discussed upthread. In reviewing the bra clasp DNA, I am more convinced than I was before that Stefanoni applied a suspect-centered approach.
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