Dubious DNA deductions deserve debunking
Kermit,
You wrote about DNA from fingerprints and from dust:
http://www.internationalskeptics.com/forums/showthread.php?postid=5799330#post5799330
You misunderstood the paper on fingerprints for a second time despite my efforts to point you in the right direction. They were enhancing the fingerprint with ninhydrin or one other chemical, DFO. Ninhydrin does not enhance the extraction or amplification of DNA; to understand how it can harm the DNA one needs to know that ninhydrin reacts with primary amines, and adenine has a primary amino group. In other words, the special treatment was for the fingerprints, and if anything, the possible reaction of ninhydrin with DNA could cause of the reduction in the amount of DNA that these authors observed. However, not every fingerprint needs visualization with ninhydrin.
Your argument that this technique is in its initial stages is wrong; people have been studying touch DNA for over ten years. The reference I supplied did indicate that not every paper surface worked equally well to yield DNA, but that is irrelevant to how Raffaele’s DNA might have been transferred from the door (try a Google Scholar search if you don’t like the references I provided). We suspect that Raffaele’s shoulder had contact with some portion of the door, and it is reasonable to suppose that his hands also had contact with the door (not necessarily the handle). All I suggested was that this contact is one of many ways his DNA might have ended up on the bra clasp by secondary or tertiary transfer.
With respect to DNA and dust, a little history is in order. I first brought up the DNA-in-dust paper to show that Stefanoni was wrong in her assertion that skin cells do not contain DNA. That Stefanoni was wrong was confirmed in the thread that Kestrel started on this topic. In this thread I showed an electropherogram (Figure 3) from the forensic DNA dust article:
http://www.internationalskeptics.com/forums/showthread.php?postid=5798634#post5798634
This is the figure to which my earlier comment alluded. You did not disappoint; you managed to muddy the waters here as well. There are two reasons why the study in question did not observe full profiles from any one individual. First, the authors used dust from classrooms and hallways; it is not a surprise that it showed contributions from many individuals. This makes the interpretation much more difficult. Second, the DNA was degraded from age and storage conditions. You can see evidence of this in the way the peak heights decline in the figure when one moves from smaller to larger DNA fragments (see Jason Gilder’s thesis, Chapter 3, for more information on how peak heights decline as the size of the DNA increases). It is not the technique of extracting DNA from dust, but the sample itself (mixed and degraded DNA) that prevented a full profile from being observed.
I did not bother to pursue the issues surrounding dust before, because I think that there are more likely means of transferring Raffaele’s DNA onto the clasp than through household dust. For example, Katy_did offered a plausible suggestion involving towels about a month ago. However, your dubious deductions from this article and the ones on fingerprints deserve debunking.