Chris_Halkides
Penultimate Amazing
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- Dec 8, 2009
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Interview with Professor Stephen Bustin on PCR
I only have a little time today to discuss quantitative polymerase chain reaction qPCR), but Professor Stephen Bustin is a world-recognized authority on the subject, having written an article that has been cited several thousand times, as well as many other articles and book chapters. In an interview he said, "Furthermore, a properly designed, optimized and validated RT-qPCR test is the most sensitive, specific, reliable and robust method for detecting a pathogen."
Regarding the issue of being "fit for purpose," Professor Bustin said, "These sentiments arise from the deliberate spreading of false information, coupled with misleading quotes and incomplete reporting. They are made by people who have little knowledge and no understanding of the technology, but are articulate and rely on the general public’s ignorance and lack of interest in scientific detail. The media also are at fault, because they have generally failed to distinguish between authoritative scientific conclusions based on data and facts versus false and unreliable opinions based on mendacity and sophistry.
"The most common falsehood is that the inventor of PCR, Kary Mullis, claimed that PCR should not be used for diagnostics. He did make an unfortunate comment about the reliability of diagnostic testing, but this related specifically to the detection of HIV in AIDS patients, was aimed at conventional gel-based PCR and was made in 1993, before real-time (qPCR) was in use as a diagnostic tool. I knew Kary Mullis personally and I know for certain that this was not his opinion as we discussed, and he was interested, in the use of RT-qPCR as a prognostic tool for colorectal cancer patients."
I only have a little time today to discuss quantitative polymerase chain reaction qPCR), but Professor Stephen Bustin is a world-recognized authority on the subject, having written an article that has been cited several thousand times, as well as many other articles and book chapters. In an interview he said, "Furthermore, a properly designed, optimized and validated RT-qPCR test is the most sensitive, specific, reliable and robust method for detecting a pathogen."
Regarding the issue of being "fit for purpose," Professor Bustin said, "These sentiments arise from the deliberate spreading of false information, coupled with misleading quotes and incomplete reporting. They are made by people who have little knowledge and no understanding of the technology, but are articulate and rely on the general public’s ignorance and lack of interest in scientific detail. The media also are at fault, because they have generally failed to distinguish between authoritative scientific conclusions based on data and facts versus false and unreliable opinions based on mendacity and sophistry.
"The most common falsehood is that the inventor of PCR, Kary Mullis, claimed that PCR should not be used for diagnostics. He did make an unfortunate comment about the reliability of diagnostic testing, but this related specifically to the detection of HIV in AIDS patients, was aimed at conventional gel-based PCR and was made in 1993, before real-time (qPCR) was in use as a diagnostic tool. I knew Kary Mullis personally and I know for certain that this was not his opinion as we discussed, and he was interested, in the use of RT-qPCR as a prognostic tool for colorectal cancer patients."
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