PRESIDENT - There are other questions for cross-examination only of course on this last point because it had already been exhausted.
PROSECUTOR DR COMFORTABLE - Yes, yes, but I'll be very brief.
PRESIDENT - Please prosecutor.
PROSECUTOR DOTT.SSA COMODI - About the latter argument is ... quell'accertamento result is negative if it is blood or other material that reacts to that test, however, to TMB but appears to have been found Amanda DNA of those tracks we're talking about?
CONSULTANT - Then aspects that I go to my slide because that memory could say things that are not correct, then we have found for example, 178 tells us that from that material was extracted a Knox profile, 179 idem, 180 idem.
PROSECUTOR DR COMFORTABLE - Here, then maybe we can not say for sure whether it's human blood or other material could however rule out the possibility of an animal as there is human DNA?
CONSULTANT - Well then back again on the human DNA that could result, not of blood, but as we said last time could come ...
PROSECUTOR DR COMFORTABLE - From biological material.
CONSULTANT - From saliva, flaking cells.
PROSECUTOR DR COMFORTABLE - Yeah sure.
CONSULTANT - That's right.
PROSECUTOR DR COMFORTABLE - Yes of course, but I say there is a given ... on the one hand there is a negative to that ...
CONSULTANT - At tetramethylbenzidine, yes to the TMB.
PROSECUTOR DR COMFORTABLE - See, in that exam there to where you could not determine what it was that material fact that has been analyzed, right?
CONSULTANT - Yes.
PROSECUTOR DR COMFORTABLE - At least it was not possible to establish that it was blood, however, say that confirmation was found Knox's DNA?
CONSULTANT - Of course, the genetic profile of Knox was found.
PROSECUTOR DOTT.SSA COMODI - Okay and about always of this TMB, this analysis at the level percentage according to his experience, this analysis made on tracks with enhanced luminol are more cases in which the analysis is negative, this type of ... it is negative or the TMB are more cases in which it is positive?
CONSULTANT - So, I guess we can say is 50 percent because sometimes luminol gives positive traces that in reality the TMB are negative and sometimes ... I would say a 50 and 50 I can neither say yes or say no on one side or the other.
PRESIDENT - I'm sorry, maybe TMB if you can give us an explanation?
CONSULTANT - E 'tetramethylbenzidine TMB, is an acronym that is ...
PROSECUTOR DR COMFORTABLE - It 'basically a reaction, what a reaction?
CONSULTANT - Yes, yes it is a clorimetrica reaction that occurs in the presence of tetramethylbenzidine, once used benzidine then they saw that as a carcinogen was better to take it off the market and therefore is used ... but it's exactly the same principle if you have present the sticks that are used to verify the presence, for example, blood in urine works in exactly the same way so we have a clorimetrica reaction.
PROSECUTOR DR COMFORTABLE - Okay, another question but maybe she has already implicitly answered because going back to S.A.L. cards It should be 'apart from his first ... his first date questions to writing ...
CONSULTANT - So it's the first slide I showed on this thing here.
PROSECUTOR DR COMFORTABLE - Data writing June 12, 2008.
CONSULTANT - Yes.
PROSECUTOR DR COMFORTABLE - The data show that brings the report of Stefanoni filed ...
CONSULTANT - If it makes me demand will be 12 ... so yeah I guess ... then connected to this point, it was ... I put what it means because it was my ... I had not realized then that's fine.
PROSECUTOR DR COMFORTABLE - And the results if there is one ... the result when did the forensic team was announced the enrollment of the suspects?
CONSULTANT - No, I do not know, at this point I guess is 12/11/2007, I guessed right?
PROSECUTOR DR COMFORTABLE - Why has a different date depending on whether the proceedings are still against persons unknown and then you do the draws against unknown persons and then ...
CONSULTANT - I noted I just could not understand this discrepancy between the two dates, now I have the answer that's great.
PROSECUTOR DOTT.SSA COMODI - then the beginning of the operations in the proceedings against it has become known.
CONSULTANT - Against known, all right.
PROSECUTOR DR COMFORTABLE - The fact that ... well about the dates in general, date missing some dates ...
CONSULTANT - Yes for example here ...
PROSECUTOR DR COMFORTABLE - Related ...
CONSULTANT - Refers to this, amplification ...
PROSECUTOR DR COMFORTABLE - Here, it should be 'well that we were correct its relief although I am told that certain operations are not exactly been made over the first, but in fact that we were correct its importance for the outcome final that miss in the SAL some shows what's involved?
CONSULTANT - But I ...
PROSECUTOR DR COMFORTABLE - That is precisely the purpose of the final result from the technical and scientific point of view because the formal aspect, administrative, legal, bureaucratic organization like saying so I mean it's something that does not pertain to its specific expertise.
CONSULTANT - Certainly not. Then we say that the relevance that I had done the lack of date regarding the quantification and capillary electrophoresis I resolved alone in that are missing here, however, we find them elsewhere and therefore are indications that we have and that we are useful examination of the documentation, examination of the inquiries that have been made, however what it lacks precisely that is that there is nowhere in all the documents that we have had access to this date is the amplifications, I now I do the example of the lab where I work, for example, we had to create a tab in the registers where, however, indicate the exact date of when it was made because the amplification so we see them also samples of different cases are amplified together just to do this check also on the possible contamination that exist in the laboratory, then to me as part of the consultant knowing or not knowing if that day were amplified ten samples that contained the DNA of the victim and this sample 36 B understands that perhaps I may be of interest, however, because as we said at the quantification Exhibit 36 B gives too low then the indication of this uncertainty in the amount of DNA which could be equal to zero understands that I doubt that there has been a contamination in subsequent operations of amplification, however, I can not verify it until I do not know which samples were amplified together.
PROSECUTOR DOTT.SSA COMODI - Okay, then these data would be served in the good substance does not in order to appreciate the goodness of the findings but in order to verify the possibility of contamination.
CONSULTANT - One and two to see if it really is none of the samples that was delivered was not amplified, for example, twice, three times because it can happen, it can happen that perhaps a reaction amplification, but I say this from personal experience is bad because maybe in that moment you are distracted, you forgot to add something, or is bad because you miscalculated, it is true that you have, however, quantify the amount of DNA is not that so ideally you choose to later boost for course always get the best result you can get is obvious that ... to know if a track is amplified many times gives me the information over and above all for those tracks that we were told of a ... contained a smaller amount of DNA to 200 picograms we said last time to be the limit for considering a low copy number trace, and then whether to ese mpio were performed ... do not speak only of the track 36 B I speak in general of all those tracks that gave results below the quantification of 200 picograms, if for example also for the amplification or not was repeated.
PROSECUTOR DR COMFORTABLE - In the papers she could look after the deposit of the end of July there are ... there is a list of metrics?
CONSULTANT - There is a list of ...
PROSECUTOR DR COMFORTABLE - Samples quantified?
CONSULTANT - Yes there is a list of quantified samples fact regarding the quantification I know exactly when they were quantified. "