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Can you get testable DNA from...um....fecal matter?

EvilSmurf

Graduate Poster
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Mar 25, 2005
Messages
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A few of my coworkers were telling me a situation that happened at this workplace about a year ago (before I had started working there). Someone defecated in an area of the plant which is not reserved for such as an activity (i.e. it was not in the restrooms, and was in an area of the plant far from the rest room). They were telling me the supervisors were threatening to DNA test the fecal matter and take DNA of everyone who worked there to find out who it was. Which led me to the question in the title of the thread. So, can you?
 
A few of my coworkers were telling me a situation that happened at this workplace about a year ago (before I had started working there). Someone defecated in an area of the plant which is not reserved for such as an activity (i.e. it was not in the restrooms, and was in an area of the plant far from the rest room). They were telling me the supervisors were threatening to DNA test the fecal matter and take DNA of everyone who worked there to find out who it was. Which led me to the question in the title of the thread. So, can you?

That doesn't really sound legal, but IANAL. DNA testing is more accurate if you have a specific suspect.

However, part of the fecal material is cells shed from the lining of your gut. It might be a bit trickier than other samples (relatively low concentration of your own cells vs. other material and microbes), but it can be done.

Linda
 
That doesn't really sound legal, but IANAL. DNA testing is more accurate if you have a specific suspect.

However, part of the fecal material is cells shed from the lining of your gut. It might be a bit trickier than other samples (relatively low concentration of your own cells vs. other material and microbes), but it can be done.

Linda

IANAL either but I'd assume if they did it on a "voluntary" basis (taking note of who didn't volunteer) it would be legal.
 
I imagine it wouldn't be cheap either, so I'd seriously doubt that it's more than a bluff.
 
Strange, but I just had a thought. I saw a case where the dad killed his young son. They found vomit in the truck and they found cereal but could not check for DNA. I don't remember details but there is something in vomit that kills DNA. I'm wondering if fecal material is the same way or not or are the cells in there not affected.
 
This happens all the time in the lab I work at (not the situation, but the testing type).
It is possible to get good DNA extracts from stools as the epithelial cells of the culprit would be deposited on the surface of the stool (assuming it's solid), but the DNA processing and analysis is expensive and not worthwhile unless the company can afford it - I doubt it would, unless they have nothing better to do with their time.
 
Strange, but I just had a thought. I saw a case where the dad killed his young son. They found vomit in the truck and they found cereal but could not check for DNA. I don't remember details but there is something in vomit that kills DNA. I'm wondering if fecal material is the same way or not or are the cells in there not affected.
 
Dang. My computer and dial up service is way too slow. My first comment was supposed to be 3rd. Sorry.
 
I presume it's probably also not that uncommon to find...hairs...from the fecal extracting area.
 
Strange, but I just had a thought. I saw a case where the dad killed his young son. They found vomit in the truck and they found cereal but could not check for DNA. I don't remember details but there is something in vomit that kills DNA. I'm wondering if fecal material is the same way or not or are the cells in there not affected.

I get a strange feeling of deja-vu with this one.
Vomit would be high in acid and enzymes, which would degrade your DNA very rapidly. I'm not sure how much "template" DNA you could get out of it to be useful for STR "DNA Finger printing" analysis and what quality it would be in.
I don't know of any procedures used for this, but in the circumstances, working out what type of breakfast cereal it was would be interesting evidence. If it matched with what was found in the victims house (depending on its popularity) could narrow down the search somewhat.
 

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