A simplified and effective method for freezing and storage of chick embryo fibroblasts at −79°C*
Stephen T. Springera, Philip C. Stiff JRa, Harold H. Leea
Purchase
a Department of Biology, The University of Toledo, Toledo, Ohio, USA
Received 9 June 1969; Available online 21 December 2006.
Summary
A simple method has been worked out forfreezing and storage of chick embryo fibroblastic cells. Cell suspensions were shelf-frozen at −75°C to −79°C, stored in the growth medium, and supplemented with 10% dimethyl sulfoxide in polypropylene tubes. More than 90% of the cells which were stored for 220 days were still viable after thawing.
*The investigation was supported in part by Grant E-553 from the American Cancer Society, Inc. to H.H.L.