Thanks Kaosium for the thoughtful reply on the DNA questions. I have suspected (without an informed basis) for awhile that the DNA test results on the bra clasp were due to over fitting. It sounds like that I was probably wrong.
It sounds like a small but significant amount of RS DNA was on the clasp. I understood, I think, the point you made about how DNA derived from a specific biological source (skin, blood, etc.) was more useful for determining the significance of a positive result than DNA from an unknown cell origin. Still a possibly reliable result that the second biggest contributor to the DNA on the clasp was RS is a surprising result.
It seems unlikely that RS DNA could have gotten there by random contamination if he was the second largest source of DNA on the clasp.
Why? Meredith's contribution was 7-10 times that of Raffaele's, what makes the fact his was three times (or so) larger than the other minor contributors all that significant? He was at the discovery, he exerted himself mere feet away from where the bra clasp was moving around. The door being broken in might have flung any traces on it into the room--and onto the floor where the bra clasp traversed somehow during the interim between the bra being collected and the return trip. DNA degrades with time, depending on the substance and the conditions. Bone DNA that's frozen might last centuries (or more!) however other DNA is not as sturdy, for example in this case the bloody towels had degraded during that six week period, the rest of the bra clasp sample would also degrade eventually due to storage. Being as we don't know what the sample is, whatever it was of Raffaele's may not have been any larger, just something that degraded slower than whatever it was that the one male who had the one allele which was higher than Raffaele's at one locus but overall lower, it could be the rest of the string had degraded, that's one sign of it.
If one just sampled the floor in Kercher's bedroom randomly how likely would it be that one would detect RS DNA? If DNA lies all over the floor willy nilly I would expect the DNA from other individuals to completely swamp out a small contribution from RS.
We'd have to see the EDFs to make that determination, that's one of the (likely) reasons they'll never be made public. Note if you go by Stefanoni's 100 RFU threshold on most every other sample tested you can't put together a profile of Raffaele on the bra clasp either.
I am bemused as the government shutdown has apparently 'degraded' some (but not all) government links, but I did find
one paper on the effects of degradation on tissue samples that includes this quote:
McCord et al 2011 p2 said:
Our overall conclusions are that
1) Environmental damage to DNA in tissue samples occurs rapidly to the point that DNA becomes nearly unrecoverable. The template in such samples breaks down to very small pieces in as little as 3 weeks.
Added to that is the fact that kerentinized skin cells don't have DNA but the ones with
sweat, say from exerting oneself or being in a panicky situation
are more likely to:
Touch DNA Suzanna Ryan p. 1 said:
The average human sheds roughly 400,000 skin cells per day (Wickenheiser, 2002); however, since it is known that the top-most layers of skin are basically “dead”, being keratinized and having lost their nuclei (Kita, et al 2007), where does the touch DNA come from? Kita, et al, performed experiments which showed that small amounts of fragmented DNA is present on the surface of the skin and they theorized that these fragments of DNA may be constantly sloughed off the keratinized cornified layer of skin and that sweat may also contain fragmented DNA. Later research by Quinones and Daniel (2011) verified that the presence of sweat helps to contribute to the DNA profile obtained from touch DNA samples.
These researchers showed that cell free nucleic acids, or CNAs, (basically free-floating DNA fragments not encapsulated in the cell nucleus) contribute greatly to the total amount of DNA present in a sample with CNAs being detected in the sweat of 80% of healthy individuals tested. They also found that, along with CNAs, nucleated cells were present in sweat samples taken from volunteers.
If you're looking for a good primer on the subject of touch DNA (it has tables and charts too--very cool!) that's a good paper to peruse and it's only about eight pages long and written in English and not bio-speak.
I don't have a feel at all what random DNA sampling on a floor is likely to detect. If one sampled my bedroom floor would one find my DNA in every or almost every sample?
Not necessarily, but to find it odds are you'd have to go looking for low template DNA, if you just maintained a 100 RFU threshold with typical sample sizes it would probably be rare, but if you lower that....
As for finding Raffaele's specifically, let me try this analogy. If I were to stand at that doorway and fling a dozen grains of salt into that room and you were to close your eyes, take a few steps forward and put your finger down at any one location odds are damned slim you'd contact one of those salt granules. However were you to move your finger three feet in one direction (the very minimum the bra clasp moved) odds are significantly better you'd contact some salt. Were you to move your finger about around and around, simulating the possibility the bra clasp was blown or kicked around it would get even better. Now if I'd dropped another dozen or so grains on the ground in front of the door and went in and out of the room several times you'd be even more likely to find some salt on your finger as you were moving it around a few feet in front of that door.
Perhaps the idea isn't that the clasp was contaminated when it was lying on the floor? Is the most likely source of contamination the function of police or forensic investigator mishandling?
That's a big one too, especially with the dirty gloves. I'm just specifically posting on this possibility as it's not as unlikely as I've seen others suggest and you seemed to be implying.
Keep in mind the bra clasp was
not the only thing they collected during the second trip, nor was it the only DNA testing they did. If someone leaves a minute amount of DNA just by
being somewhere (and doing nothing involved in the murder) the best way to find it is test as many things as possible. Now if the sample was something actually
damning it would be something else entirely, but what I'm saying is that finding trace DNA from someone at a site they'd been at, from an item that had 'been around' and had at the minimum have moved around on the floor several feet is not particularly indicative of that item having actually been touched by that person, and the lack of other indications of his presence
on or around the body where they tested extensively (the bra clasp was not originally found on the body itself) makes it rather anomalous in regards to suggesting he was involved in the murder.
Is there other testing that might have been done to either rule out or rule in the RS DNA on the clasp as evidence that RS touched the bra clasp? If RS DNA was on the clasp because he actually touched it as part of the crime against Kercher would one expect to find his finger prints on the clasp? Or perhaps one would expect to find much more of his DNA on the cloth part of the bra which would have been expected to scrape off some of skin cells?
Fingerprints, no. Those don't actually adhere very well and finding a usable one on the bra clasp would have been practically impossible considering its tiny size. Incidentally you've probably never heard one bad word from
innocentisti regarding ILE's fingerprint work because they did outstanding work there, they didn't produce dubious partials that could be misinterpreted and when identifying Rudy Guede from the handprint they nailed it.
It would be more likely that his DNA would have been found on the cloth covering if he'd tried to remove the bra clasp, but considering that the bra was (likely) ripped off from the front bending the clasp and tearing the fabric to the point it would probably have been hanging from a thread given it was found near the bra but came detached shortly after, odds are no one outside Meredith actually touched that clasp, Rudy Guede and her boyfriend were excluded according to one of the experts, Tagliabracci if I recall correctly.
Incidentally that touch DNA study mentions another FBI study that Halides1 has linked regarding the prevalence of transfer DNA on someone's underwear. If Meredith had a habit of adjusting the clasp as it would twist and become uncomfortable or something, she might well have transferred DNA of people around her to the clasp and that's where that DNA came from.
I'm sure all this was discussed in great detail previously and I appreciate the patience of anybody who can perhaps rehash a bit of what as gone before on this.
No problem, you're more fun to post to than Dr. Tesla was! I don't mean to damn with faint praise either, even though it might have come across that way, it was merely ironic understatement.
