http://www.sciencespheres.com/2010/03/lcn-dna-part-ii-watch-where-you-sneeze.html
And from my post this morning:
Amanda's appeal
Looks to me like she just turned up the "volume".
That last quote may indicate she also did what you suggest, which would make her results even more suspect, I would suspect.
Just to follow up on my previous post, the details of the testing of the knife I read (a while ago now) from Massei report translation, the discussion is spread out over 100+ pages, specific citations from pg 255, defense expert Dr. Gino:
It did, however, emerge from the documentation that some of the samples taken from Exhibit 36 were quantified with the Qubit fluorometer, and that the results for some of them were "too low". Thus the quantity was inferior to the base value (or threshold setting) of the kit; it could even have been equal to zero. This result, she asserted, should have led to cessation of the analysis.
Qubit fluorometerWP, as the wiki mentions, "uses fluorescent dyes to determine the concentration of nucleic acids and proteins in a sample", from what I gather this was machine which was tweaked because of the "too low" results.
Pg 258, of the report:
Returning to Exhibit 36, she specified that she had focused on trace B and not on
trace A and the profile which emerged from it.
She stated that in the hearing before the GUP, Dr. Stefanoni had spoken of a quantity equal to a few hundred picograms; from later documentation it appeared that in fact, it was a quantity labelled "too low", and, she observed, such a quantity cannot amount to a few hundred picograms (page 88). However, it appeared to her that, Dr. Stefanoni (again, before GUP) specified that she had performed the PCR at 28 cycles; in fact, she recalled that she had been asked why "if it was a case of a low copy number sample, she had not amplified it with more than 28 cycles, as usually suggested in the guidelines" (page 88).
PCR amplification of 28 cycles is the standard amplification, so even though the amount of DNA was LCN, the DNA profile was not extracted through extra amplifications associated with "LCN testing" method, so any contamination would of shown up in subsequent standard tests.
Last edited: