Again, I don't think they are strict biological influences, but more societal overlays on top of biological differences but if you want a rough outline...
From an early age boys are expected to be physical, aggressive, competitive, more badly behaved. Because that's what 'boys' are like. So you are expected to play sports and fight in the playground, and if you are the kind of boy who likes to read poetry then you are a cissy, or gay.
Once they hit puberty they are expected to be walking sacks of hormones and penises. Success is defined by how many girls you can sleep with which also drives a lot of behaviour around establishing dominance, being ultra competitive, having to be ripped and a lot of insecurities if you don't happen to meet the desired stereotypes of being tall, muscly and big-dicked. If you prefer to stay home, study and write short-stories then you are a virgin loser.
Move on a bit and the same stuff is in play but now you are also supposed to establish yourself as a 'powerful' man in business/work life. God forbid you want to be a carer or work with kids - then you are probably a paedo because you have a penis. In all situations you are a potential rapist and have to treat yourself as such.
And as an adult you have to walk the tightrope of simultaneously being both a powerful, successful, assertive, sexually-dominant alpha-male while also acknowledging that all of this is also toxic masculinity and undesirable and you are expected to settle down, have kids and be a loving, caring, father despite never having been prepared for that by society. Don't overdo it though because you still have to be a 'man'.
Then being a male kills you sooner than being a female either because all of the above BS stresses your heart to the point where it konks out, or your prostate/balls get cancer or you never get to that point because you sat in your car with the exhaust piped into the cabin one day just to get away from it all.
And that's not to come over all 'woe is me' about being a man. We have it good in a number of ways as well. But these 'biology' things works both ways. What I do think though is that a transwoman is not going to experience the same things as a cisman growing up just because they have penises and balls. And from the moment they start transitioning that experience is going to diverge.
So if we only look at biology then we are missing something major - because if you want to insist that transwomen are not women because they have a penis and testicles and don't have the experience of being women then I'm going to say that they are also not men simply because they have a penis and testicles without the experience of being a man.
So again this biological essentialism completely fails to recognise the reality of society.