A study of common interferences with the forensic luminol test for blood
Quickenden and Creamer
Department of Chemistry, University of Western Australia
Luminescence 2001;16:295–298
ABSTRACT: A wide range of domestic and industrial substances that might be mistaken for haemoglobin in the forensic luminol test
for blood were examined. The substances studied were in the categories of vegetable or fruit pulps and juices; domestic and
commercial oils; cleaning agents; an insecticide; and various glues, paints and varnishes. A significant number of substances in each
category gave luminescence intensities that were comparable with the intensities of undiluted haemoglobin, when sprayed with the
standard forensic solution containing aqueous alkaline luminol and sodium perborate. In these cases the substance could be easily
mistaken for blood when the luminol test is used, but in the remaining cases the luminescence intensity was so weak that it is unlikely
that a false-positive test would be obtained. In a few cases the brightly emitting substance could be distinguished from blood by a
small but detectable shift of the peak emission wavelength. The results indicated that particular care should be taken to avoid
interferences when a crime scene is contaminated with parsnip, turnip or horseradish, and when surfaces coated with enamel paint are
involved. To a lesser extent, some care should be taken when surfaces covered with terracotta or ceramic tiles, polyurethane varnishes
or jute and sisal matting are involved. Copyright 2001 John Wiley & Sons, Ltd.
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Those disagreeing with the work of the above or the Minnesota Department of Public Safety (https://dps.mn.gov/divisions/bca/bc...es/forensic-programs-crime-scene-luminol.aspx) should let those professors or law-enforcement agencies know their views.
The above is only applicable if a spectrometer is used (as in this study) to analyse the light output from Luminol, Steffanoni did no such thing.