I've found another online copy of the English translation of the Conti-Vecchiotti Report. This one has the advantage of being a continuous document available on one web page:
Now that I can refer to a simpler page-numbered format on a single web page, I will point out some issues and pose some questions. Note that RTIGF is an Italian acronym for Technical Report on the Forensic Genetic Tests and SAL is an acronym for Work Status Report (a card format).
1. Page 53. Stefanoni had to remove presumed material from the sample points on the knife. The proper way to do so, minimizing the risk of contamination, would include certain measures such as bleaching work surfaces before beginning the removal, wearing sterile clean gloves that would be changed between samples, and using sterile swabs and other sterile instruments. However, Stefanoni did not record any of this activity and the measures taken to minimize contamination during this procedure.
2. Page 54. Stefanoni's SAL and RTIGF show that she performed a presumptive test for blood and an antibody test for any trace of human tissue on samples 36 B, C, E, and G (all from the knife blade). Both tests were negative for each sample.
3. Page 55. Stefanoni did not record any tests or microscopic observations to look for cells or cell fragments in those samples, so it must be assumed that none were attempted, but she continued to presume the presence of biological material in samples 36 B, C, E, and G, and began the procedures preparatory for DNA testing.
4. Page 55. The RTIGF records that an automated extractor was used to extract the presumed DNA from the samples; the SAL records that samples 36 A, B, and C were DNA-extracted on 13 November 2007, while samples 36 D, E, F, and G were DNA-extracted on 17 December 2007. The extraction volume was 50 microlitres (ul) for each sample. (I'm using "u" for the Greek letter mu for convenience.)
5. Page 56. The RTIGF shows that the DNA concentrations were found by Real Time PCR using a 7700 Sequence Detector ABI PRISM™ apparatus
from the company Applied Biosystems. The RTIFG record shows that samples 36 A and B were positive for DNA, but without any numerical value for the quantity found, while sample 36 C, D, E, and F were negative for DNA. No information on the kit used for the quantification was provided in the record. However, the records for the Real Time PCR show that it was used only for samples 36 C, D, E, and F on 18 December 2007, and indeed, they all tested negative (0.00) for DNA .
6. Pages 57 - 58. For samples 36 A, B, and C, there is another report attached, dated 13 November 2007, that shows those samples were tested with a Qubit Fluorometer from the company Invitrogen using the kit dsDNA HS. The range of accuracy for this fluorometer is a DNA concentration of 0.2 to 100 ng/ul. The fluorometer found the following DNA concentrations, according to Stefanoni's written record: sample 36 A, 0.08 ng/ul; 36 B, too low [to be measured], 36 C, too low [to be measured]. Even though the actual quantification showed only A had measurable DNA, Stefanoni continued after the fluorometer quantification with the original (unmeasured) claim that both samples A and B had measurable DNA.
7. Page 59. Stefanoni, during the GUP hearing (4 October 2008) testified that sample 36 B had DNA that had been quantified using Real Time PCR and that the amount of DNA was “in the order of some hundreds of picograms [pg]”, "a value which does not appear in any of the documents provided" to C&V. [100 pg = 0.1 ng (nanograms)] The amount in sample 36 B had to be far less than that in 36 A, which had 4 ng = 4000 pg, and might have contained no DNA whatsoever.
Question 1: It appears that Stefanoni falsified her records and her testimony in the GUP hearing, claiming to have used Real Time PCR to quantify samples 36 A, B, and C, but in reality using a fluorometer to determine the quantification. She did not correct her original statement in her written records. Why would she falsify her records and her testimony? What does that imply about the credibility of the data? Was Stefanoni justified in claiming that sample 36 B had "some hundreds of picograms" of DNA if the fluorometer could not determine the amount because of its limited sensitivity? Why was she satisfied that sample 36 C had no DNA, because it show "too low", but treated sample 36 B as having DNA although it gave the same reading as 36 C?