How can any size be attributed to the Singularity? Given it represents all of existence, what else is it being measured against? Can it somehow be measured against itself internally?
The big bang singularity is present in all the solutions of Einstein's equation that describe a homogeneous and isotropic universe. All of those solutions either describe an expanding universe, or a universe with a non-zero density of vacuum. Astronomical observations showed that the universe is expanding (light from other galaxies is redshifted), and that the density of vacuum is at least very close to zero. The original big bang theory is just the claim that our universe can be represented mathematically by one of those solutions.
One of those solutions is easier to understand than the others, so I'll focus on that one. It describes space, at any given time, as a three-sphere with radius R(t), where t is the time coordinate. The time coordinate is always positive in this solution. The function R is increasing, and also goes to 0 as t goes to 0.
That last thing is what physicists really mean when they say that this solution contains a singularity. The singularity is not a point or a region in space-time, so it can't have a size. However, since the "radius" of the universe goes to zero as time goes to zero, the size of the universe can be made arbitrarily small by going far enough back in time. A somewhat sloppy way of saying this would be to say that the size of the universe was 0 at t=0, but there really is no t=0 in this solution. It would be even sloppier to say that the size of the singularity is zero, but I suppose even that would be OK in a conversation between two people who already understand what I've said here.
Is the Universe that expanded from the Singularity still a Singularity? The internal structure may have changed, but isn’t it still a single entity that represents all of existence?
As I said, the time coordinate is always positive in these solutions, so the universe didn't expand from t=0. The universe has never
been a singularity in this theory, because there never was a t=0.
Do we know the size of the Universe now (if so, how), and how do we know that it’s not infinite?
We don't know if it's infinite or not.