The 'Mixed Blood' and Forensic Sophistry with Trace DNA
Here is what the prosecution tried to pretend was Amanda's blood mixed with Meredith's in the sink. You won't find that contention in Massei, in fact you'll get the impression from him quoting Stefanoni that they
didn't make that contention, as she admits therein that it cannot be determined with the tests they did, but in court, which Massei does not mention, they did in fact 'hypothesize' that the samples were mixed blood even through she had to make that admission under questioning.
Here is forensic sophistry at it's finest. First look at the collection method, review
this video, specifically around 1:35 if you don't want to sit through the whole four minutes. Note how he's swabbing the sink, he doesn't just collect the tiny little traces of Meredith's blood that were left there when Rudy washed up, he swabs far and wide nearby and another forensic technician points out something he missed and he rubs down the edge of the sink as well for good measure. Also of interest is the swabbing of the bidet drain which starts at about 3:30.
That was the sink (and bidet) that Amanda and Meredith shared, if you watched the whole video you may have noticed directly before 1:30 the technician was swabbing the dried blood on the tap from Amanda's earring, which as you'll see from Massei was mixed with nothing and even Massei allows that it had been there before as he details in the excerpt below. That's hardly all that was in (or on) that sink that was unrelated to the murder, also there is quite likely
plenty else that had been in that sink and deposited there the previous two months, or at least that which had survived previous cleanings, the (barely in this video it can be seen better
here at 3:55) visible blood smear hardly the only thing that might be in that sink from her brushing her teeth every day and spitting in it. As for the bidet drain, that would tend to collect traces of whatever was being washed out, which caused even Luciano Garafano to protest, and he'd been an ally of Mignini when he was trying to find the devil by digging up bodies.
A portion of those swabs would then go into a little tube and the DNA extracted with chemicals. Stefanoni used the
Qiagen EZI for which I linked the manual and a video showing it,
here's a page showing another such machine with a less commercial (but quite
boring) video showing an extraction from a mouse tail (poor little mouse!) taking the place of the swab and the loading of the chemicals that perform the extraction.
Here's a lower tech way of extracting DNA using household chemicals from a PBS Nova video.
So as you can see, what's on that swab is going to get mixed together, and being as Meredith's blood was on that swab (and it tested positive in a separate blood test) along with anything that might be in Amanda's sink, most likely saliva containing cheek cells, (this may be the most common way of collecting DNA incidentally--swabbing cheek cells) that extraction sample is going to contain both Meredith's DNA and Amanda's. After extraction that sample will be amplified (to make it easier to 'count') and the DNA then 'sequenced' through
capillary electrophoresis and then analyzed and lo and behold we have two electropherograms for the
sink and
bidet with both Amanda and Meredith's profiles on it.
So there's actually no reason to think that DNA (of Amanda's) was ever actually in the same spot as the minute quantify of blood of Meredith's, or has any connection to the murder, any more than the dried blood droplet from Amanda's endeavor to turn her ears into pincushions does. This is what they are referring to when they say they found 'mixed DNA.' It does not mean that the DNA was deposited at the same time either, if they swab like that it most certainly doesn't even mean it was in the same spot, just that it was picked up by the same swab. Of all the places you're most likely to find usable DNA samples
at any time a person's sink has to be amongst the best, people spit in sinks while brushing their teeth, they wash their face and hands, they brush their hair, apply make up and use hygiene products in front of the mirror.
Now here's Giancarlo Massei on the subject of Amanda's blood on the tap from her ear (which mixed with nothing) and the mixed DNA samples and the contention of 'mixed blood.' As the verdict was guilty, and this was evidence of Meredith's death being as Rudy Guede washed up in there and left traces of Meredith's blood around, Massei had to try to attribute what he could to the defendants on trial, Raffaele and Amanda. However even he choked on the idea of 'mixed blood' and instead tried to use the mixed DNA samples as evidence that Amanda had washed up with Meredith's blood on her and by scrubbing vigorously left her skin cells in Meredith's blood. However, he also noted this before starting:
Massei PMF 278-9 said:
It is true that, according to what was asserted and explained, it is not possible with a mixed trace specimen that tested positive for human blood to determine which of the trace’s contributors the blood belongs to.
He also acknowledged this rather important point which directly follows the above:
Massei PMF 279 said:
In this case, however, non-mixed traces were also found, which were shown to be of a haematological nature [i.e. blood] and turn out to have the biological profile of the victim. Such traces, in particular the dribble of blood left on the right inside edge of the door and the stains left on the light switch (see photographic illustrations 141, 142; 158, 159) lead to the deduction that whoever entered that bathroom had his or her hands covered in Meredith’s blood.
Furthermore, the sky-blue bathmat with the print of a bare foot in blood, blood which also was shown to be from the victim, indicates that whoever went into this bathroom was barefoot, and must therefore also have been barefoot in Meredith’s room where she had been repeatedly struck, a room which had great blotches of blood, and in one of these whoever transferred the blood to the bathroom and the sky-blue bathmat must have placed his or her foot, and thus must have been moving about that room with bare feet.
The above observation leads to the deduction that whoever went into the bathroom at that point (after the stabbing of Meredith) must have had to do so to clean him/herself of Meredith’s blood with which he/she was staining the various things he/she touched or leaned against: the door, the light switch, the mat. And it is probable - not necessary, but probable - that during the following act of scrubbing the hands to remove the blood, he/she left the mixed trace consisting of Meredith’s blood and of cells which had been removed by rubbing during the act of washing. An entirely probable outcome given the likelihood of the act of scrubbing, yet not a necessary one, since the running water which was used in the shower stall or in the bidet or in the sink, or in several of these sanitary fittings, might well have rinsed away the washed-up blood and the cells which had been lost during this washing.
That's one reason why there's no identified traces of Rudy in the bathroom, even though he was the one who left his DNA all over Meredith's room and it is certain that he had Meredith's blood on him, especially his hands and probably feet. He had to have cleaned that up before leaving (he'd have looked strange dancing with bloody hands and feet) and of course most of the traces amount to someone having touched the light switch, cotton box and door frame with bloody hands, and the bathmat bloodstain sure looks more like his foot than Raffaele's and I'm more skeptical of the 'measurements' taken by the prosecution 'expert' that seem to require some fourth dimensional space in order to add up as noted by Rose recently on the
Conspiracy thread.
Cleaning up once quickly isn't nearly as good an opportunity to leave plentiful DNA traces as two or so months of activity doing many things that don't involve cleaning oneself. Despite the fact that throughout this section he's trying to attribute these traces to Amanda being as though they collected Amanda's DNA in her bathroom, he's handicapped by the fact that
in reality one cannot pretend that one quick use of a bathroom will necessarily leave copious traces, especially if that activity is cleaning oneself up. He also has to recognize the fact that DNA doesn't come with a timestamp either, and quotes Stefanoni on that very point, as she too had to admit to that in court, along with with the fact that because that swab with Meredith's blood tested positive for blood that hardly means that all the DNA was due to blood, any more than Rudy's DNA inside Meredith means it came from Rudy's vaginal fluids.
As for Amanda's (non-'mixed') dried blood smear on the tap, he refuses to attempt to use that as evidence against her, and again we see when Massei doesn't have to attempt to support an unlikely contention, he is actually a pretty clear and logical thinker:
Massei PMF 279-280 said:
While it is not possible to use the genetic scientific data (Dr. Stefanoni explained the impossibility of determining the date, the succession or the simultaneity in the depositing of the components of the mixed trace specimen and the impossibility of attributing the haematological component to one or the other of the contributors), the information previously put forward provides answers which are entirely consistent with the circumstantial evidence that has emerged and which the Court considers convincing.
Amanda was not wounded; in the days following no one spoke of wounds that she might have had; the examination which was carried out on her when measures restricting her personal freedom were taken ruled out the presence of wounds. Meredith’s situation was the complete opposite. In relation to this and to the circumstance by which haematological stains attributable to Meredith were found on the inside of the door, on the toilet-seat cover, on the light switch, it should be deduced that the haematological components found in the sink, in the bidet, on the box of cotton buds were also from Meredith.
Nor can it otherwise be argued for the presence of a drop of Amanda’s blood on the tap of the sink. This consisted of a spot of coagulated blood, with respect to which Amanda explained that it came from her own ear having been pierced; this spot, furthermore, was located towards the inside of the sink: distinct, separate and morphologically different, therefore, from the trace found in the sink itself.
In other words there's no reason to think the dried blood on the tap was involved in the murder, unlike the other tiny droplets that were collected in the video above that smear had coagulated, suggesting it had been there longer, and of course there's one very good reason to think it wasn't: Amanda was not wounded, and it's not like they didn't check most thoroughly to see if she had been.
There was one person who most definitely got Meredith's blood on him and needed to clean up, there's one person who's foot seems to match that bathmat stain better. That person would be
male and this is a bathroom mainly (in fact almost exclusively) used by
females. That means one could use the Y-haplotype test to determine if there was any DNA that could be attributed to a male that might be masked by the almost certain robust traces of the women that
lived there and used the bathroom on a daily basis, just like they did on the bra-clasp and how they often do in rape cases. Why didn't they do that?
However, and more importantly, why didn't they go looking for LCN/LT trace DNA in
this location? One doesn't need to rely on LCN/LT trace DNA to find copious amounts of DNA evidence in the bathroom of someone who
lives there, it's pretty much a given that you're going to find it if you look hard enough and swab far and wide. However, if they were going to go looking for LCN/LT trace DNA in this case, there's two definite locations that should have been included, as there's people out there who
just don't get that if one doesn't go
looking for LCN/LT trace DNA they're not going to
find any. Here's why:
AmpFISTR Identifiler manual p. 42 said:
Peak amplitude thresholds – The software uses these parameters to specify the minimum peak height, in order to limit the number of detected peaks. Although GeneMapper® ID Software displays peaks that fall below the specified amplitude in electropherograms, the software does not label or determine the genotype of these peaks.
From the
GeneMapper manual:
GeneMapper Manual p.247 said:
Peak AmplitudeThresholds
GeneMapper software reports to the user only those peaks whose heights are at least the Peak Amplitude Threshold for that dye.
Set the dye amplitude threshold at a level that allows the software to detect peaks, but eliminate noise.
If you leave the default value of 50, peaks with amplitude above 50 are analyzed and display in the tabular data.
Lower amplitude peaks still display in the electropherogram, but are not analyzed and do not display in the tabular data.
Stefanoni chose a Peak Amplitude Threshold of 100 for almost all the DNA analysis outside a few selected items, mainly the bra clasp, the knife blade and a few others here and there. However that's where one is going to find the trace elements of DNA, if one doesn't even set the perameters to
look for it (except to give some
special treatment) you cannot find it.
Now look at the bottom of some of
these electropherograms from the bathroom (that I haven't already posted). Make sure to look to the left as a reference, that's the RFU's and on typical samples they aim for 1k-2k. Those little bumps at the bottom registering roughly fifty, or a hundred RFUs are where you'd go looking for the trace DNA. So they not only have to do the forensic work at the scene, they have to analyze it in the lab and determine to count it. To give a comparison, most the 'peaks' (a generous term!) on
36B (knife blade) are between 20 and 40 RFUs, quite a bit lower than many of the little bumps at the bottom of the bathroom samples.
If they don't collect it, (samplewise) they didn't look, if they set the threshold so as to not recognize it, they cannot find it either. Anyone expecting trace DNA results from anywhere they didn't look or didn't try to find isn't taking into account the fact those sorts of trace elements can only be found that way. Obvious substances with plentiful DNA were collected as best they could, however trace elements from the window in Filomena's room and the bathroom were damned unlikely to be found with a threshold of 100 RFUs.
Therefore in the case of the bathroom and the break-in window 'absence of evidence is
not evidence of absence.' Someone merely passing through or cleaning up once is not guaranteed to leave plentiful DNA about, however that
might be found if looking for trace elements, but you cannot find find them if you do not try. They looked very hard in the murder room and found plentiful samples of someone whom there's very little reason to believe could have an innocent explanation of them, they found utterly nothing of Amanda Knox no matter how hard they tried and for Raffaele only something they contaminated on camera for all the world to see, if it wasn't enough they left it there to perhaps skitter around the floor like a dust bunny for six weeks. That means they tried
hard and didn't get anything useful, however where they very likely
could have found trace elements that...let's just say...they 'didn't need to make their case,' there's little interest in pursuing peaks of less than 100 RFUs. Of course without the
electronic data files one cannot really look at those little bumps and see what they might be, and whose profile they might spell out, those would actually allow a full
analysis of those cute little bumps:
Jason Gilder courtesy of View From Wilmington Chris Halkides 4/5/10 entry said:
“Each tested sample has its own file. The file contains the full electropherogram trace information along with other information about the testing conditions (e.g., date, time, injection time, voltage, temperature, current, the RFU threshold used by the analyst). If you have the electronic data, you can use the DNA analysis software (GeneScan & Genotyper or GeneMapper ID) to independently analyze the electronic data. That allows you to examine the results as closely as possible (zoom in on the electropherogram to evaluate low-level results) and establish the RFU threshold of your choosing."
Stefanoni fought tooth and nail to avoid providing that information to the defense or even the independent experts assigned to the court.
Forensic sophistry at it's finest!