Here is everywhere Massei talks about the box that the knife delivered to Rome in:
Massei - [99] (Google translation)
He then reported that the return to the police station Inspector Finzi handed material seized in the home of Sollecito Raffaele. The first thing was finding the knife that was inside a new envelope that was wrapped well and it appeared so close that had no contact with the outside world (p. 201 and 223). Clear that when the knife was finding new gloves, which he had used on other occasions and that he had taken office. So, with these gloves, removed the knife from the bag and put it inside a box sealed with tape. Stated that the box previously and there were no other agenda items if not "new agenda donated" by a bank (p. 202). This box was then sent, along with other findings, the Scientific Police in Rome (p. 203).
Massei - [224]
Stated again that this knife, exhibit 36, had received in a box and was delivered in the laboratory where he was photographed and analyzed. The container of the knife is not satisfied that it was sterile, but stated that "even the remains are sterile ..
Massei - [272]
What was achieved was considered so due to contamination and this for a number of reasons: how repertoire (knife findings by the forensic team in Perugia and then transferred to Rome within an envelope and a box of cardboard ;
Massei - [282]
Exhibit 36.
That knife was the first object that touched and was above all the dishes. Put it in a paper bag, new, and he was carrying and then in a folder. The envelope with the knife handed in to the superintendent Gubbiotti qundo returned to police headquarters.
In turn, the superintendent Gubbiotti ... received the knife Finzi: the object was inside a well sealed envelope new . Gubbiotti also stated that when the knife was finding new gloves, never used before and had taken office. It was with these new gloves that he took the knife from the bag and put it in a box sealed with scotch and transmitted it to other findings in the Scientific Police in Rome...
Massei - [283]
...when he pulled out the envelope in which to place it was kept in a box that previously contained a new agenda, a gift of ima bank and sealed, conveyed to the Scientific Police in Rome.
She stated that when finding that the knife had new gloves that he had used on other occasions on which, therefore, could not find the DNA that he would be found on the knife. In the box where the knife was placed certainly can not think of that might be contaminating DNA of Amanda and Meredith because it was a box which was kept a diary new box that is sealed, was sent to Science in Rome.
Only the one entry on page [272] talks about the knife being in both the box and an envelope. I would seriously think that Massei would not talk so much about the box if the knife were isolated from the box by being inside an envelope.