Kaosium
Philosopher
- Joined
- Oct 12, 2010
- Messages
- 6,695
Thanks for bringing attention to the uncalled alleles! This e-gram makes very clear why "Doctor" Stefanoni would not release the EDFs - there is a lot of DNA evidence and/or contamination hiding in the electronic data files that she dares not reveal.
Exactly, those edfs will never be seen, Machiavelli expects they'll be destroyed as soon as the ISC rules, proving in this instance that the crime is worse than the cover-up.
To my eye, roughly estimating, the heights of the uncalled allele peaks in the 177 e-gram are about 100 RFU - well above the often used 50 RFU limit for noise.
Yes, and note from the electropherogram I (belatedly--had to edit it in because I forgot to link it initially) posted for the knife blade that most those alleles didn't even clear 50 RFUs, one is about 18 RFUs!
Lab Tech "Doctor" Stefanoni used her own never-validated LCN DNA method for the knife blade to call alleles when it suited the prosecution's purpose, and it would not be surprising if there were low levels of contamination DNA detectable in many of the EDFs.
Yep, and if we could get our hands on those edfs along with reference profiles for Stefanoni, the forensic technicians, the others at the discovery like Filomena and her boys as well as the postales then we could prove it. However Nencini refused to allow the edfs to be seen due to the bogus argument (or half truth) that the edfs couldn't prove contamination for an individual sample, however it could prove endemic contamination which is a huge danger doing low template work in a lab not suited for it and using procedures which weren't even followed correctly for high template work.
Bascially with low template work you're looking for contamination level DNA, thus you shouldn't be surprised to find it if you don't follow the protocols.