Chris_Halkides
Penultimate Amazing
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Response with respect to luminol, Part B
Point 13. Every test costs money, and if you follow luminol with TMB, you are spending more money and time than if you just use luminol. Your answer makes no sense; therefore, let’s see what forensic chemists have to say. In “Biological Evidence Collection and Forensic Blood Identification,” Castro and Coyle said, “It is advised that if a positive reaction with luminol is achieved, the stained area should be checked again with another reagent such as tetramethylbenzidine, phenolphthalein, or orthotolidine and always confirmed with a human-specific confirmatory test for blood [23].”
“Either luminol or fluorescein can be sprayed onto large surfaces such as walls or floors and the positive areas areas marked for further testing. Both tests are very sensitive and will indicate bloodstains that may not be visible…One disadvantage to these tests is that both can have false positive reactions. Luminol and fluorescein will react with the same false positives as PH [phenolphthalein] and also with bleach and other cleaning fluids, which may interfere with blood detection on surfaces that have been cleaned. For this reason fluorescein or luminol positive areas should be retested with one of the color change presumptive tests.”
The Forensic Laboratory Handbook Procedures and Practice (Humana Press, 2006)
Chapter 1 Serology and DNA, by Lisa A. Gefrides, NS and Katherine E. Welch, MS
Point 6. The correct answer is that a positive result with HemaTrace or the RSID test would do so. Given that HemaTrace can detect blood diluted to levels equal to or beyond what luminol can detect, there is no excuse not to use it. The plausible substances include iron and manganese from soil, iron from rust, and manganese from plant matter.Again it's irrelevant. There is no tests that ever "ruled out" a specific substance, but in evidence assessment process, there is no need to rule out specific substances. The point is that the burden is reversed: there is a need to have a plausible alternative substance and a plausible alternative dynamic, in order to undermine and dismiss the piece of circumstantial evidence.
A link between luminol prints and blood is already expressed by the analogy of features and same context of the luminol prints with the bathmat bare footprints in diluted blood; also, as well as by the peculiarity of the traces themselves, by the coincidence with one of Guede's shoeprints and by other information such as Knox's statements.
And there is no plausible substance containing metal ions. No such substance was detected inside the flat (and all bottles and substances present are recorded); the substances containing metal salts are usually toxic and not for direct human use (enamels, varnishings, gardening products) while the prints are of bare feet. Those alternative substances would react to TMB as well, but they are just not probable nor plausible, there is no evidence of their existence anywhere in the house, and there is no plausible or known dynamic for how a pattern of prints with that set of peculiar features would have been produced in any metal based substance.
Point 7. They are coauthors of a review on forensic testing of body fluids. I have quoted them many times. I asked them about the areas that were negative for TMB or DNA. “So, there was either no blood and the luminol was wrong, or there was blood and the TMB had interference and the luminol damaged the DNA. We think it is more likely that there was no blood, and that the luminol was reacting with something else, possibly plant matter from the bottom of the shoes causing the footprints (the intensity of the luminol reaction might give some more insight). The prosecution should have used much more convincing evidence to prove the presence of blood.”I have no clue of what Virkler and Lednev said.
Point 8. When a spot is in the wrong place, one has to explain both its presence and the absence of a spot where it should be. Your explanation fails to explain who there is no spot where the tip of Ms. Knox’s toe should be. It is a track, not a print, and Rinaldi’s supposed expertise is a slender reed on which to lean. Dr. Vinci showed how wrong Rinaldi was about the shoe prints in the murder room. Why should anyone take Rinaldi as an expert, given his dismal track record?I don't know why the "second toe" doesn't match, but I can infer that if it's Knox's print, it might be the mark from a drop and not from direct contact. I can also note that the "second toe" in question was never discussed with prosecution's witnesses: when Rinaldi testified, he matched the print to a probable identity with Knox, while none of the defence questioned him about the "second toe".
Obviously it makes no sense to split the question 'whether it is blood' from the rest of the questions. It's worth recall that several prints however can't be matched with sufficient reliability with anyone, but anyway three of them are in Knox's room.
Point 9. Some of the foot tracks in the hallway are supposed to be female and some male. Did both Amanda and Raffaele do this?!Because the person/s was/were dragging himself/herself on a bathmat and/or an equivalent piece of fabric.
Point 10. My working hypothesis is that luminol reacted to old foot tracks unrelated to the crime. These foot tracks might have been created when iron or manganese on the sole of a foot bonded with the flooring. How the overapplication of luminol might be related to this is an interesting question. Luminol is reported to be able to penetrate through up to 8 layers of paint.No, it's impossible. The overapplication itself can give autoluminescence as a residual effect, but cannot give to autoluminescence the shape of human feet.
Point 11. This is just wrong. Any time a supposed expert makes a mistake, it impacts their credibility, as it should, within reason.No, not at all.
Point 12. Could it be animal blood? If it were Meredith’s blood, then why isn’t Meredith’s DNA present? Your answer to point 1 is unconvincing and is pure speculation.Blood.
All tests serve the same purpose: collect as more information as possible, when it's possible.
The reason why TMB is suggested (by some, but not all authors) is not because it is colorimetric, but because it is cheap and easy, it can be safely recorded. While luminol is better, it has also inconvenients: it is rather difficult to use and what happens if it goes wrong? Like if the photographies are not good enough or if the preparation is bad. Better have a cheap and safe second test, so you still have evidence in any event.
But aftr all, still this question is irrelevant.
Point 13. Every test costs money, and if you follow luminol with TMB, you are spending more money and time than if you just use luminol. Your answer makes no sense; therefore, let’s see what forensic chemists have to say. In “Biological Evidence Collection and Forensic Blood Identification,” Castro and Coyle said, “It is advised that if a positive reaction with luminol is achieved, the stained area should be checked again with another reagent such as tetramethylbenzidine, phenolphthalein, or orthotolidine and always confirmed with a human-specific confirmatory test for blood [23].”
“Either luminol or fluorescein can be sprayed onto large surfaces such as walls or floors and the positive areas areas marked for further testing. Both tests are very sensitive and will indicate bloodstains that may not be visible…One disadvantage to these tests is that both can have false positive reactions. Luminol and fluorescein will react with the same false positives as PH [phenolphthalein] and also with bleach and other cleaning fluids, which may interfere with blood detection on surfaces that have been cleaned. For this reason fluorescein or luminol positive areas should be retested with one of the color change presumptive tests.”
The Forensic Laboratory Handbook Procedures and Practice (Humana Press, 2006)
Chapter 1 Serology and DNA, by Lisa A. Gefrides, NS and Katherine E. Welch, MS
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